Lee Lab - Specialist

A specialist opportunity in flow cytometry and immunology research is available at the University of California, San Francisco in the Lee Laboratory. The Lee Lab works on cancer immunotherapy is part of the Department of Radiation Oncology and Department of Medicine - Hematology/Oncology.

We are a lab focused on cancer immunotherapy research, including discovery of new immunotherapy resistance pathways that make cancer immunotherapy less effective usinbg preclinical and clinical data. We will also test new treatments to overcome resistance mechanisms.

This specialist will assist with flow cytometry, clinical and preclinical specimen preparation for cytometry, cell sorting, and 10X RNA seq, cell culture, preclinical mouse research (including surgical procedures), molecular biology and immunology in a new translational cancer research laboratory at UCSF with a focus on next generation cancer immunotherapeutics. The candidate will support the lab in using multiparameter flow cytometry, high-throughput RNAseq, spatial transcriptomics, and other cutting-edge techniques with clinical sample analysis, develop novel animal models to conduct mechanistic studies with parallel patient sample analysis, and test preclinical innovative immunotherapy strategies with CAR T cells, stereotactic radiation, and mouse metastasis surgery. Specifically, the specialist's tasks will include the support for the lab's flow cytometry, clinical and preclinical specimen reception/preparation for cytometry, T cell culture, mouse research colony handling, molecular biology (RT-PCR, CRISPR, cloning, gel eletrophoresis, transfections, DNA/RNA extraction for transcriptomic studies, chip arrays, cellular culture/screens, etc), flow cytometry data analysis, and clinical tissue procurement and processing for the studies.

The specialist will be trained by the Lee Lab for relevant tasks if needed and also be able to take advantage of other training and learning opportunities within the UCSF Helen Diller Family Comprehensive Cancer Center and other related entities at UCSF. We hope this experience will prepare the applicant for future opportunities in biomedical research.

Required Qualifications:

* Junior - Specialists appointed at the junior rank must possess (or in process of obtaining) a baccalaureate degree (or equivalent degree) in biology, immunology, bioinformatics, computer science, data science, computational biology, or a related quantitative/scientific discipline or at least four years of research experience (e.g., with instrumentation and research equipment, social science research methods, or creative activities).

* Assistant - Specialists appointed at the Assistant rank must possess (or in process of obtaining) a master's degree (or equivalent degree) or a baccalaureate degree in biology, immunology, bioinformatics, computer science, data science, computational biology, or a related quantitative/scientific discipline with 3 or more years of research experience.

* Associate - Specialists appointed at the Associate rank must possess (or in process of obtaining) a master's degree (or equivalent degree) or five to ten years of experience in the relevant specialization.

* Full - Specialists appointed at the full rank must possess (or in process of obtaining) a terminal degree (or equivalent degree) or ten or more years of experience in the relevant specialization.

* Strong Flow Cytometry technical background, including sample preparation, multiparameter FACS, cell sorting, and Flowjo analysis.

* Strong problem-solving skills

* Strong communication, data presentation, and writing skills

Preferred Qualifications:

* Over 2 years of experience with flow cytometry and its data analysis

* Over 2 year of wet lab experience

* Experience with mouse colony maintenance

* Experience with IHC and FFPE preparation

* Experience with clinical and preclinical tumor / tissue sample processing

Please apply online at https://aprecruit.ucsf.edu/JPF04703. Applicants' materials must list current and/or pending qualifications upon submission.

See Table 24B for the salary range for this position. A reasonable estimate for this position is $51,300-$181,800.

• 2-4 required (contact information only)

As a University employee, you will be required to comply with all applicable University policies and/or collective bargaining agreements, as may be amended from time to time. Federal, state, or local government directives may impose additional requirements.

UC San Francisco seeks candidates whose experience, teaching, research, or community service has prepared them to contribute to our commitment to diversity and excellence. The University of California is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age or protected veteran status.